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chip dna clean concentrator kit  (Zymo Research)


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    Structured Review

    Zymo Research chip dna clean concentrator kit
    Chip Dna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+dna+clean+concentrator+kit/ChIP+DNA+Clean+%26+Concentrator/pmc13109728-100-15-21
    Average 99 stars, based on 1513 article reviews
    chip dna clean concentrator kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Cell-free chromatin epigenomic profiling enables non-invasive pancreatic cancer cell-state identification
    Article Snippet: Subsequently, beads were resuspended and incubated in 100 μL of DNA extraction buffer containing 0.1 mol/L NaHCO3, 1% SDS, and 0.6 mg/mL Proteinase K (Qiagen, cat #19131) and 0.4 mg/mL RNaseA (Thermo Fisher Scientific, cat #12091021) for 10 minutes at 37°C, for 1 hour at 50°C and for 2 hours at 65°C. .. DNA was purified using the ChIP DNA Clean & Concentrator Kit (Zymo Research, cat #D5205). .. Cell-free ChIP-seq (cfChIP-seq) libraries were prepared with ThruPLEX DNA-Seq Kit (Takara Bio, cat #R400675) following the manufacturer’s instructions.

    Article Title: Bloom syndrome helicase is required for efficient HIV-1 reverse transcription in macrophages
    Article Snippet: Following antibody binding, extracts were incubated with pAG-MNase, 1 mM calcium chloride and then the reaction was stopped and cross-linking reversed using 0.1% final SDS (Cell Signaling Technology, #20533) and 20 μg/mL proteinase K (Cell Signaling Technology, #10012). .. DNA, including input samples, was purified using ChIP DNA Clean & Concentrator kit (Zymo Research, #D5205). qPCR was performed using primers designed to target HIV-1 U5 LTR, primer binding site (pbs), psi and cPPT regions. .. Control primers targeted human RPL30 gene (Cell Signaling Technology, #7014) and GAPDH (Thermo). (all primers are in )

    Article Title: RUNX1 and YY1 modulate neuronal fate and energy metabolism in Alzheimer’s disease
    Article Snippet: .. Collected nuclei were then resuspended in 50 μL transposase reaction mix (1x Tagment DNA buffer, 2.5 μL Tagment DNA enzyme I in water (Illumina), and incubated at 37°C for 30 min. DNA was purified with a Zymo ChIP DNA Clean & Concentrator Kit (Zymo Research D5205) according to the manufacturer’s instructions. .. DNA was then amplified with PCR mix (1.25 μM Nextera primer 1, 1.25 μM Nextera primer 2-bar code, 0.6x SYBR Green I (Life Technologies, S7563), 1x NEBNext High-Fidelity 2x PCR MasterMix, (NEBM0541) for 7-10 cycles.

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. DNA was purified using DNA Clean & Concentrator kit (Zymo Research, D4034) for inputs and ChIP DNA Clean & Concentrator kit (Zymo Research, D5205) for ChIP samples. .. Purified DNA concentration was measured with a Qubit 4 Fluorimeter (Thermo Fisher Scientific, Q33238 ) using the QubitTM double-stranded DNA (dsDNA) HS Assay Kit (Thermo Scientific, Q32854 ) for ChIP DNA and the QubitTM dsDNA BR Assay Kit (Thermo Scientific, Q32853 ) for input samples.

    Article Title: Tau-induced elevation in promoter-proximal RNA polymerase II pausing is linked to decreased expression of long neuronal genes in a Drosophila tauopathy model
    Article Snippet: .. DNA was purified using the Zymo ChIP DNA Clean & Concentrator Kit (#D5205), and libraries were prepared with the EpiCypher CUTANA Library Preparation Kit (#14-1001). .. The libraries were pooled and sequenced in a single lane using paired-end 150-bp reads on an Illumina Novaseq X Plus platform.

    Article Title: Transcription Factor Subtype Governs Response and Resistance to DLL3-Directed T-Cell Engagement in Small Cell Lung Cancer
    Article Snippet: Subsequently, the beads were rinsed with TE buffer (Thermo Fisher Scientific, Cat. #BP2473500) and resuspended and incubated in 100LJμl of DNA extraction buffer containing 0.1LJM NaHCO3, 1% SDS and 0.6LJmgLJml−1 Proteinase K (Qiagen, Cat. #19131) and 0.4LJmgLJml−1 RNaseA (Thermo Fisher Scientific, Cat. #12091021) for 10LJmin at 37LJ°C, for 1LJh at 50LJ°C and for 90LJmin at 65LJ°C. .. DNA was purified according to the manufacturer protocol using the ChIP DNA Clean & Concentrator kit (Zymo Research, Cat. #D5205). cfChIP-seq libraries were prepared with ThruPLEX® DNA-Seq Kit (Takara Bio, Cat. #R400675) following the manufacturer’s instructions. .. After library amplification, the DNA was purified by AMPure XP (Beckman Coulter, A63880).

    Chromatin Immunoprecipitation:

    Article Title: Cell-free chromatin epigenomic profiling enables non-invasive pancreatic cancer cell-state identification
    Article Snippet: Subsequently, beads were resuspended and incubated in 100 μL of DNA extraction buffer containing 0.1 mol/L NaHCO3, 1% SDS, and 0.6 mg/mL Proteinase K (Qiagen, cat #19131) and 0.4 mg/mL RNaseA (Thermo Fisher Scientific, cat #12091021) for 10 minutes at 37°C, for 1 hour at 50°C and for 2 hours at 65°C. .. DNA was purified using the ChIP DNA Clean & Concentrator Kit (Zymo Research, cat #D5205). .. Cell-free ChIP-seq (cfChIP-seq) libraries were prepared with ThruPLEX DNA-Seq Kit (Takara Bio, cat #R400675) following the manufacturer’s instructions.

    Article Title: Bloom syndrome helicase is required for efficient HIV-1 reverse transcription in macrophages
    Article Snippet: Following antibody binding, extracts were incubated with pAG-MNase, 1 mM calcium chloride and then the reaction was stopped and cross-linking reversed using 0.1% final SDS (Cell Signaling Technology, #20533) and 20 μg/mL proteinase K (Cell Signaling Technology, #10012). .. DNA, including input samples, was purified using ChIP DNA Clean & Concentrator kit (Zymo Research, #D5205). qPCR was performed using primers designed to target HIV-1 U5 LTR, primer binding site (pbs), psi and cPPT regions. .. Control primers targeted human RPL30 gene (Cell Signaling Technology, #7014) and GAPDH (Thermo). (all primers are in )

    Article Title: RUNX1 and YY1 modulate neuronal fate and energy metabolism in Alzheimer’s disease
    Article Snippet: .. Collected nuclei were then resuspended in 50 μL transposase reaction mix (1x Tagment DNA buffer, 2.5 μL Tagment DNA enzyme I in water (Illumina), and incubated at 37°C for 30 min. DNA was purified with a Zymo ChIP DNA Clean & Concentrator Kit (Zymo Research D5205) according to the manufacturer’s instructions. .. DNA was then amplified with PCR mix (1.25 μM Nextera primer 1, 1.25 μM Nextera primer 2-bar code, 0.6x SYBR Green I (Life Technologies, S7563), 1x NEBNext High-Fidelity 2x PCR MasterMix, (NEBM0541) for 7-10 cycles.

    Article Title: Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activator
    Article Snippet: .. DNA was purified using DNA Clean & Concentrator kit (Zymo Research, D4034) for inputs and ChIP DNA Clean & Concentrator kit (Zymo Research, D5205) for ChIP samples. .. Purified DNA concentration was measured with a Qubit 4 Fluorimeter (Thermo Fisher Scientific, Q33238 ) using the QubitTM double-stranded DNA (dsDNA) HS Assay Kit (Thermo Scientific, Q32854 ) for ChIP DNA and the QubitTM dsDNA BR Assay Kit (Thermo Scientific, Q32853 ) for input samples.

    Article Title: Tau-induced elevation in promoter-proximal RNA polymerase II pausing is linked to decreased expression of long neuronal genes in a Drosophila tauopathy model
    Article Snippet: .. DNA was purified using the Zymo ChIP DNA Clean & Concentrator Kit (#D5205), and libraries were prepared with the EpiCypher CUTANA Library Preparation Kit (#14-1001). .. The libraries were pooled and sequenced in a single lane using paired-end 150-bp reads on an Illumina Novaseq X Plus platform.

    Article Title: Transcription Factor Subtype Governs Response and Resistance to DLL3-Directed T-Cell Engagement in Small Cell Lung Cancer
    Article Snippet: Subsequently, the beads were rinsed with TE buffer (Thermo Fisher Scientific, Cat. #BP2473500) and resuspended and incubated in 100LJμl of DNA extraction buffer containing 0.1LJM NaHCO3, 1% SDS and 0.6LJmgLJml−1 Proteinase K (Qiagen, Cat. #19131) and 0.4LJmgLJml−1 RNaseA (Thermo Fisher Scientific, Cat. #12091021) for 10LJmin at 37LJ°C, for 1LJh at 50LJ°C and for 90LJmin at 65LJ°C. .. DNA was purified according to the manufacturer protocol using the ChIP DNA Clean & Concentrator kit (Zymo Research, Cat. #D5205). cfChIP-seq libraries were prepared with ThruPLEX® DNA-Seq Kit (Takara Bio, Cat. #R400675) following the manufacturer’s instructions. .. After library amplification, the DNA was purified by AMPure XP (Beckman Coulter, A63880).

    Real-time Polymerase Chain Reaction:

    Article Title: Bloom syndrome helicase is required for efficient HIV-1 reverse transcription in macrophages
    Article Snippet: Following antibody binding, extracts were incubated with pAG-MNase, 1 mM calcium chloride and then the reaction was stopped and cross-linking reversed using 0.1% final SDS (Cell Signaling Technology, #20533) and 20 μg/mL proteinase K (Cell Signaling Technology, #10012). .. DNA, including input samples, was purified using ChIP DNA Clean & Concentrator kit (Zymo Research, #D5205). qPCR was performed using primers designed to target HIV-1 U5 LTR, primer binding site (pbs), psi and cPPT regions. .. Control primers targeted human RPL30 gene (Cell Signaling Technology, #7014) and GAPDH (Thermo). (all primers are in )

    Binding Assay:

    Article Title: Bloom syndrome helicase is required for efficient HIV-1 reverse transcription in macrophages
    Article Snippet: Following antibody binding, extracts were incubated with pAG-MNase, 1 mM calcium chloride and then the reaction was stopped and cross-linking reversed using 0.1% final SDS (Cell Signaling Technology, #20533) and 20 μg/mL proteinase K (Cell Signaling Technology, #10012). .. DNA, including input samples, was purified using ChIP DNA Clean & Concentrator kit (Zymo Research, #D5205). qPCR was performed using primers designed to target HIV-1 U5 LTR, primer binding site (pbs), psi and cPPT regions. .. Control primers targeted human RPL30 gene (Cell Signaling Technology, #7014) and GAPDH (Thermo). (all primers are in )

    Incubation:

    Article Title: RUNX1 and YY1 modulate neuronal fate and energy metabolism in Alzheimer’s disease
    Article Snippet: .. Collected nuclei were then resuspended in 50 μL transposase reaction mix (1x Tagment DNA buffer, 2.5 μL Tagment DNA enzyme I in water (Illumina), and incubated at 37°C for 30 min. DNA was purified with a Zymo ChIP DNA Clean & Concentrator Kit (Zymo Research D5205) according to the manufacturer’s instructions. .. DNA was then amplified with PCR mix (1.25 μM Nextera primer 1, 1.25 μM Nextera primer 2-bar code, 0.6x SYBR Green I (Life Technologies, S7563), 1x NEBNext High-Fidelity 2x PCR MasterMix, (NEBM0541) for 7-10 cycles.

    DNA Sequencing:

    Article Title: Transcription Factor Subtype Governs Response and Resistance to DLL3-Directed T-Cell Engagement in Small Cell Lung Cancer
    Article Snippet: Subsequently, the beads were rinsed with TE buffer (Thermo Fisher Scientific, Cat. #BP2473500) and resuspended and incubated in 100LJμl of DNA extraction buffer containing 0.1LJM NaHCO3, 1% SDS and 0.6LJmgLJml−1 Proteinase K (Qiagen, Cat. #19131) and 0.4LJmgLJml−1 RNaseA (Thermo Fisher Scientific, Cat. #12091021) for 10LJmin at 37LJ°C, for 1LJh at 50LJ°C and for 90LJmin at 65LJ°C. .. DNA was purified according to the manufacturer protocol using the ChIP DNA Clean & Concentrator kit (Zymo Research, Cat. #D5205). cfChIP-seq libraries were prepared with ThruPLEX® DNA-Seq Kit (Takara Bio, Cat. #R400675) following the manufacturer’s instructions. .. After library amplification, the DNA was purified by AMPure XP (Beckman Coulter, A63880).

    other:

    Article Title: Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation.
    Article Snippet: CUT&RUN was performed using CUT&RUN Assay Kit (Cell SignalingTechnology) and antibody against H3K27ac (Cell Signaling Technology, 1:50).



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